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KMID : 0375319940160010175
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Journal of Clinical Pathology and Quality Control
1994 Volume.16 No. 1 p.175 ~ p.180
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A Study for Quantitation of Rubella Antibody (IgG) by Using Enzyme Immunoassay
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Abstract
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Background:
A quantitation of rubella antibody is necessary to define immune status following vaccination because the success of the vaccination in eliminating congenital rubella syndrome depend on the persistence of antibody throughout the child-bearing
age of
vaccinees.
Methods:
We quantified rubella antibody by EIA (EnzygnostTM Anti-Rubella-Virus/IgG, α-method) according to age on 198 samples at the Ewha Univ. Mokdong Hospital from Feb. to Mar., 1994.
Results:
The coefficient of variation in within-run assay and between-run assay for quantitation of rubella antibody were 13.9% and 9.1%, respectively. The median level of rubella antibody showed the highest level in 1-2 age group(after vaccination)
and
gradually decreased with the lowest level in teen age group. in 20-30 age group, the level of rubella antibody showed an increasing tendency.
Conclusions:
The alpha-method in quantitating the rubella antibody had moderate degree of precision and also had economical benefit because it could be quantitated with a single diluted specimen. The median value in teen age group showed the lowest level,
which
was probably due to the fact that immune antibodies acquired from vaccinations in the early childhood decreased with time. Therefore, a booster vaccination would be recommended at this time.
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KEYWORD
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